The Growth of Chlorella sp. With Varying Nutrient Concentration

  • Intanurfemi Hismayasari Politeknik Kelautan dan Perikanan Sorong
  • Ernawati Ernawati Politeknik Kelautan dan Perikanan Sorong
  • Agung Setia Abadi Politeknik Kelautan dan Perikanan Sorong
  • Asthervina Widyastami Puspitasari Politeknik Kelautan dan Perikanan Sorong
Keywords: Chlorella sp, growth performance, natural food

Abstract

The aim of this study was to determine the effect of differences addition of culture medium concentrations on the growth of microalgae type Chlorella sp. Samples were obtained from the Center for Brackish Water Cultivation Fisheries (BBPBAP), Jepara. This research was conducted at the Laboratory of Nutrition and Natural Feed Sorong Marine and Fisheries Polytechnic with an experimental method using four treatments with addition of walne medium, there were A (adding only at the beginning of cultivation); treatment B (adding every day); C (adding every two days), and treatment; D (adding every three days). The cell growth performance was significantly found in treatment C with the addition of nutrients every two days with a peak cell density of Chlorella sp was 2,628,450 cells / ml. There is a need for mass culture  assay to determine the level of effectiveness and efficiency.

References

Ajijah, N., Tjandra, B. C., Hamidah, U., & Sintawardani, N. (2020). Utilization of tofu wastewater as a cultivation medium for Chlorella vulgaris and Arthrospira platensis. In IOP Conference Series: Earth and Environmental Science (Vol. 483, No. 1, p. 012027). IOP Publishing. https://iopscience.iop.org/article/10.1088/1755-1315/483/1/012027/pdf

Cahyonugroho, O. H., Yuniawati, D. D., & Hidayah, E. N. (2020). Kinetics of Chlorella sp. growth models in reducing CO2 emission. Rasayan Journal of Chemistry, 12(4), 2306-2310.

Champenois J, Marfaing H, Pierre R. Review of the taxonomic revision of Chlorella and consequences for its food uses in Europe. Journal of Applied Phycology. 2014; 27: 1845–1851.

Chilmawati, D., & Suminto, S. (2008). Penggunaan media kultur yang berbeda terhadap pertumbuhan Chlorella sp. Saintek Perikanan: Indonesian Journal of Fisheries Science and Technology, 4(1), 42-49. https://ejournal.undip.ac.id/index.php/saintek/article/view/6791

Das, P., Lei, W., Aziz, S. S., & Obbard, J. P. (2011). Enhanced algae growth in both phototrophic and mixotrophic culture under blue light. Bioresource Technology, 102(4), 3883-3887.

Fradique, Ḿonica, Batista, A. P., Nunes, M. C., Gouveia, L., Bandarra, N. M., & Raymundo, A. (2010). Incorporation of Chlorella vulgaris and Spirulina maxima biomass in pasta products. Part 1: Preparation and evaluation. Journal of the Science of Food and Agriculture, 90(10), 1656–1664. https://doi.org/10.1002/jsfa.3999

https://www.sciencedirect.com/science/article/pii/S0961953412000669

Hutami, F. E, Supriharyono, Haeruddin (2015). Laju Filtrasi Kerang Hijau (Perna viridis) Terhadap Skeletonema costatum pada Berbagai Tingkat Salinitas. Journal of Management of Aquatic Resources, 4(1), 125-130. https://ejournal3.undip.ac.id/index.php/maquares/article/view/7823

Juhl, A. R., & Murrell, M. C. (2005). Interactions between nutrients, phytoplankton growth, and microzooplankton grazing in a Gulf of Mexico estuary. Aquatic Microbial Ecology, 38(2), 147-156.

Kawaroe, M., Prartono, T., Sunuddin, A., Sari, D. W., & Augustine, D. (2019). Mikroalga potensi dan pemanfaatannya untuk produksi bio bahan bakar. PT Penerbit IPB Press. https://books.google.com/books?hl=id&lr=&id=AI8SEAAAQBAJ&oi=fn d&pg=PP1&dq=nutrien+untuk+mikroalga&ots=Hx3k30_A_c&sig=Y5run Q6tITMZpOQMvZM rVuXaHw0

Lin, T. S., & Wu, J. Y. (2015). Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition. Bioresource Technology, 184, 100–107. https://doi.org/10.1016/j.biortech.2014.11.005

Moazami N, Alireza A, Reza R, Mehrnoush T, Roghieh E, and Ali S N 2012 Biomass and Bioenergy 39 449

Purnamawati, F. S., Soeprobowati, T. R., & Izzati, M. (2014). Potensi Chlorella vulgaris Beijerinck Dalam Remediasi Logam Berat Cd Dan Pb Skala Laboratorium. Bioma : Berkala Ilmiah Biologi, 16(2), 102. https://doi.org/10.14710/bioma.16.2.102-113

Puspitasari, Asthervina Widyastami; Arfiati, Diana; Shao-Yang Hu. 2019. Can Cinnamaldehyde Increase The Innate Immune System on Medaka Larvae?. Bioinformatics and Biomedical Research Journal, 2 (1), 21-36. http://dx.doi.org/10.11594/bbrj.02.01.04

Rahardini, R. A., Helmiati, S., & Triyatmo, B. (2018). Effect of inorganic fertilizer on the growth of freshwater Chlorella sp. IOP Conference Series: Earth and Environmental Science, 139(1). https://doi.org/10.1088/1755-1315/139/1/012005

Ramaraj R, Dussadee N, Whangchai N, Unpaprom Y. Microalgae biomass as an alternative substrate in biogas production. International Journal of Sustainable and Green Energy. Special Issue: Renewable Energy Applications in the Agricultural Field and Natural Resource Technology. 2015; 4: 13–19.

Ramaraj, R., Unpaprom, Y., & Dussadee, N. (2016). Cultivation of green microalga, Chlorella vulgaris for biogas purification. IJNTR, 3, 117-122.

Samaee, S. M., Atashbar Kangarloei, B., Noori, F., & Estévez, A. (2021). An Endeavor to Find Starter Feed Alternatives and Techniques for Zebrafish First-Feeding Larvae: The Effects on Viability, Morphometric Traits, Digestive Enzymes, and Expression of Growth-Related Genes. Zebrafish, 18(1), 73-91.

Şirin, S., & Sillanpää, M. (2015). Cultivating and harvesting of marine alga Nannochloropsis oculata in local municipal wastewater for biodiesel. Bioresource technology, 191, 79-87. https://www.sciencedirect.com/science/article/abs/pii/S0960852415006203

Umainana, M. R., Mubarak, A. S., & Masithah, E. D. (2019). Pengaruh Konsentrasi Pupuk Daun Turi Putih (Sesbania grandiflora) Terhadap Populasi Chlorella Sp. Journal of Aquaculture and Fish Health, 8(1), 1-7.

Unpaprom Y, Tipnee S, Ramaraj R. Biodiesel from green alga Scenedesmus acuminatus. International Journal of Sustainable and Green Energy. Special Issue: Renewable Energy Applications in the Agricultural Field and Natural Resource Technology. 2015; 4: 1–6.

Yaakob, M. A., Mohamed, R. M. S. R., Al-Gheethi, A., Ravishankar, G. A., & Ambati, R. R. (2021). Influence of nitrogen and phosphorus on microalgal growth, biomass, lipid, and fatty acid production: an overview. Cells, 10(2), 393.

Published
2021-12-06
How to Cite
Hismayasari, I., Ernawati, E., Agung Setia Abadi, & Asthervina Widyastami Puspitasari. (2021). The Growth of Chlorella sp. With Varying Nutrient Concentration. Jurnal Airaha, 10(02), 295 - 301. https://doi.org/10.15578/ja.v10i02.280
Section
Articles