Aplication of Seaweed Gracilaria verrucosa as Biocontrol Agent in Polyculture Vaname Shrimp Litopenaeus vannamei to Prevent Infection of Vibrio harveyi

  • Ardana Kurniaji Politeknik Kelautan dan Perikanan Bone
  • Anton Anton Politeknik Kelautan dan Perikanan Bone
  • Yunarty Yunarty Politeknik Kelautan dan Perikanan Bone
Keywords: Biocontrol, polyculture, seaweed, shrimp, Vibrio harveyi

Abstract

This study aimed to examine polyculture of seaweed and vaname shrimp in laboratory scale to prevent V. harveyi infection. Study was conducted with a completely randomized design by 3 treatments and 3 repetations. Aplication of seaweed were 800 grams (P8), 400 grams (P4) and without seaweed (control). Challenge test was carried out for 10 days with bacteria concentration of 106 cfu/ml/ind in 10 shrimp using aquarium containing 100 liters of sea water. The result showed that polyculture of seaweed and shrimp could not maintain the survival rate of shrimp significantly but could suppress the population of V. harveyi in waters. Survival rate respectively from control, P4 and P8 were 60.0%, 73.3%, 76.7%. Population of bacteria in treatment was lower than control on 10th days after seaweed stocking until challenge test. Aplication of seaweed could also accelerate shrimp recovery time from bacterial infections observed in clinical symptoms that return to normal on 9th days after challenge test. Total hemocyte count (THC) were better observed in shrimp cultured seaweed on 10th days after challenge test, and differential hemocytes count (DHC) were better on 5th days after challenge test. Polyculture of seaweed and shrimp has potential to improve shrimp health status.

References

Aguirre-Guzman, G., Vazquez-Juarez, R., Ascencio, P. (2001). Differences in the susceptibility of american white shrimp larval substages (Litopenaeus vannamei) to four vibrio species. Jurnal Invert Pathol, (78): 215-219.

Apriliani, M., Sartijo, A.H., Haditomo, C. (2016). Keanekaragaman agensia penyebab vibriosis pada udang vaname (Litopenaeus vannamei) dan sensitivitasnya terhadap antibiotic. Jurnal of Aquaculture Management and Technology, 5(1): 98-107.

Austin, B. dan Austin, D.A. (2007). Bacterial fish pathogen: disease in farmed and wild fish. John Willey and Sons Ltd, England. 90 p.

Bansemir, A.M., Blume, S., Schroder, Lindequist, U. (2006). Screening of cultivated seaweed for antibacterial activity against fish pathogenic bacteria. Aquaculture, 252: 79-84.

Bell, T. A. and Lightner, D. V. (1988). A handbook of normal penaeid shrimp histology. The World Aquaculture Society, Baton Rouge, 114 pp

Beny, M.P. (2003). Tanaman rumput laut, memanen devisa. Trobos, Majalah Agribisnis Peternakan Perikanan dan Hobi Satwa, 46: 74-75.

Cano, G.A., Bourne, D.G., Hall, M.R., Owens, L., Hoj, L. (2009). Molecular identification, typing and tracking of Vibrio harveyi in aquaculture systems: current methods and future prospects. Aquaculture, 287: 1-10.

Daniels, C.L., Merrifield, D.L., Boothoryd, D.P., Davies, S.J., Factor, J.R. and Arnold, K.E. (2010). Effect of dietary Bacillus spp and mannan oligosaccharides (MOS) on European lobster Homarus gammarus L larvae growth performance, gut morphology and gut microbiota. Aquaculture, 304: 49-57.

Desrina, A., Taslihan, Ambariyanto dan Suryaningrum, S. (2006). Uji keganasan bakteri vibrio pada ikan kerapu macan (Epinephelus fuscoguttatus). Ilmu Kelautan, 11(3):119-125.

Huang, H.X., Liu, J., Xiang, P., Wang. (2013). Immune Response of Litopenaeus vannamei after Infection with Vibrio harveyi. Aquaculture, 406-407: 115-120.

Jithendran, K.P., M. Poornima, C.P., Balasubramanian, S., Kulasekarapandian. (2010). Diseases of Mud Crabs (Scylla spp): an Overview. Indian J. Fish., 57(3): 55-63.

Johansson, M.W., Söderhäll, K. 1985. Exocytosis of the prophenoloxidase activating system from crayfish haemocytes. Journal of Comp Physiol, 156 B:175–181.

Kementerian Kelautan dan Perikanan [KKP]. (2011). Kelautan dan Perikanan Dalam Angka. Jakarta. 118 hlm.

Kurniaji, A., Idris, M., Muliani. (2019). Uji daya hambat ekstrak daun mangrove (Sonneratia alba) pada bakteri Vibrio harveyi secara in vitro. Jurnal Sains Teknologi Akuakultur, 3(1): 1-9.

Lavilla-Pitogo, C.R., G.D. Lio-Po, E.R., Cruz-Lacierda, E.V., Alapide-Tendencia, L.D., De La Pena. 2000. Disease of Peneid Shrimps in the Philippines. 2nded., Southeast Asian Fiheries Development Center, Philippines., 96 p.

Lay, B.W. (1994). Analisis Mikroba di Laboratorium. PT. Raja Grafindo Persada. Jakarta. 168 hal.

Liu, C,H,, Chen, J.C. (2004). Effect of ammonia on the immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus. Fish & Shellfish Immunology, 16: 321-334.

Rahmaningsih, S. (2012). Penerapan teknologi penggunaan rumput laut sebagai biofilter alami air tambak untuk mengurangi serangan penyakit pada udang vannamei (Litopenaeus vannamei). Jurnal Teknologi Perikanan dan Kelautan, 3 (1): 11-16.

Rudi, M., Sukenda, Wahjuningrum, D., Pasaribu, W., Hidayatullah, H. (2019). Seaweed extract Gracillaria verrucosa as an antibacterial and treatment against Vibrio harveyi infection of Litopenaeus vannamei. Jurnal Akuakultur Indonesia, 18 (2): 120-129.

Samocha, T.M., A. Braga, V., Magalhães, B., Advent and T.C. Morris. (2013). Ongoing studies advance intensive shrimp culture in zero-exchange biofloc raceways. Global Aquaculture Advocate, March/April (38-41).

Siregar, A.F., Sabdono, A., Pringgenies, D. (2012). Potensi antibakteri ekstrak rumput laut terhadap bakteri penyakit kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcusluteus. Journal Of Marine Research, 1(2): 152-160.

Supono. (2011). Studi perbandingan keragaan udang windu (Penaeus monodon) dan udang putih (Litopenaeus vannamei) pada tambak semi plastik. Pena Akuatika, 3 (1) : 1-8

Suryati, E., Gunarto, Sulaeman. (2006). Analisis bioaktif tanaman mangrove yang efektif mereduksi penyakit bakteri pada budidaya udang windu. Jurnal Riset Akuakultur, 1 (1): 96-104.

Svobodova, Z., Vykusova, B. (1991). Manual for international training course on freshwater fish diseases and intoxications: diagnostics, prophylaxis and therapy.ftp://ftp.fao.org.

Syahid, M., Subhan, A., Armando. (2006). Budidaya udang organic secara polikultur. Penebar Swadaya. Jakarta; 75 hal.

Taslihan, A., Ani, W., Retna, H., S.M. Astuti. (2004). Pengendalian Penyakit Pada Budidaya Ikan Air Payau, Direktorat Jenderal Perikanan Balai Besar Budidaya Air Payau Jepara.

Thornqvist, P.O., Johansson, M.W., Soderhall, K. (1994). Opsonic activity of cell adhesion proteins and b-1,3-glucan-binding proteins from two crustaceans. Dev Comp Immunol, 18:3-12.

Trianto, Agus, W., Edi, Suryono, S., dan Rahayu S. (2004). Mangrove leaf extract Aegiceras corniculatum as antibakteril on Vibrio harveyi and Vibrio parahaemolyticus. Jurnal Ilmu Kelautan. Universitas Diponegoro. Semarang, 9 (4): 186-189.

Van de Braak, C.B.T., M.H.A. Botterblom, E.A. Huisman, J.H.W.M. Rombout, W.P. W. Van der Knaap. (2002). Preliminary study on haemocyte response to white spot syndrome virus infection in black Tiger Shrimp Penaeus monodon. Disease of Aquatic Organism, 51(2):149±155.

Yunarty, Yuhana, M., Widanarni. (2016). Effects of microencapsulated symbiotic administration at different dosages against heavy co-infection of white spot disease (WSD) and Vibrio harveyi in pacific white shrimp (Litopenaeus vannamei). Ilmu Kelautan, 21(4): 169-176.

Published
2020-12-15
How to Cite
Kurniaji, A., Anton, A., & Yunarty, Y. (2020). Aplication of Seaweed Gracilaria verrucosa as Biocontrol Agent in Polyculture Vaname Shrimp Litopenaeus vannamei to Prevent Infection of Vibrio harveyi. Jurnal Airaha, 9(02), 137 - 141. https://doi.org/10.15578/ja.v9i02.170
Section
Articles